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Looking Deep into the Tissue


Multiphoton Microscopy without Compromise

With the LSM 710 NLO Laser Scanning Microscope, Carl Zeiss is providing basic and biomedical research with improved femtosecond multiphoton technology which enables neurobiologists, developmental biologists, immunologists and plant biologists to examine complex biological systems without compromise. The special hallmarks of the LSM 710 NLO are the acquisition of high-resolution images of fluorescent structures in living animals and thick tissue specimens, long-time observation of development processes and functional imaging in combination with the photo manipulation of fluorescent structures. The high signal-to-noise ratio provided by the system ensures brilliant imaging of cellular and sub-cellular details even in deep-lying tissue layers. For the first time, up to five fluorescence signals can now be recorded simultaneously in what is called the non- descanned mode.

The basis of the new LSM 710 NLO system is extremely sensitive, non-descanned detectors for multiphoton microscopy featuring optimized optics, electronics and detector positioning. Furthermore, a special version of the non- descanned detector with GaAsP technology and signal decoupling directly on the objective lens results in a further doubling of sensitivity. Depending on the microscope stand, up to five non-descanned detectors can be used in reflected and transmitted light and operated simultaneously. Further benefits include the scanning module’s optimized transmission over the entire wavelength range up to 1100 nm and the possibility of combining the multiphoton laser with any other laser line, including lasers with emission in the near UV range. As in the configuration with non-descanned detectors, the filters can also be combined by the customer as required. Complete integration of the system control into the ZEN 2008 software platform ensures efficiency and maximum specimen protection.


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