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Zymutest/Elisara tPA-PAI-1 kit from Aniara


Zymutest/Elisara tPA-PAI-1 Elisa from Aniara only $499.
Controls included.


The ELISARA tPA-PAI-1 kit is a two-site immuno-assay for measuring complexes of human tissue plasminogen Activator (tPA) with its major inhibitor PAI-1, in plasma or in any fluid where tPA-PAI-1 complexes can be present.


In a first step, the diluted tested plasma or biological fluid is introduced into a microwell coated with a highly purified monoclonal antibody specific for human tPA. When present, tPA PAI-1 complexes are captured onto the solid phase through the tPA moiety.

Following a washing step, the immunoconjugate, which is an anti-PAI-1 monoclonal antibody coupled to horse radish peroxidase (HRP), is introduced, and binds to its specific epitope on PAI-1 present in tPA-PAI-1 complexes. Following a new washing step, the peroxidase substrate, Tetramethylbenzidine (TMB) in presence of hydrogen peroxide (H2O2), is introduced and a blue colour develops. The colour turns yellow when the reaction is stopped with Sulfuric Acid. The amount of colour developed is directly proportional to the concentration of human tPA-PAI-1 complexes in the tested sample.

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