Aniara is pleased to announce the coming release of our new unique Factor V Leiden kit.
Aniara is pleased to announce the coming realease of the New Hemoclot Kit for Measuring the APC Resistance associated with Factor V-L Quantitatively.
HEMOCLOT Quanti. V-L (product code ACK065L)kit is a clotting method proposed for measuring Factor V-L (Factor V Leiden) concentration in citrated plasma, by its resistance to the action of Activated Protein C (APC). The assay is performed in the presence of Activated Protein C. In presence of APC and Protein S (in excess), the prolongation of clotting time is directly related to the concentration of Normal Factor V, and inversely related to the amount of Factor V-Leiden (mutation R506Q).
Assay characteristics
•Quantitative reagent for measuring FactorV-L.
•No interference of plasma factor deficiencies (Other than that of FactorV).
•Excellent discrimination between Heterozygous,Homozygous and Normals.
•Single test method performed with only a ClottingTime (CT)
Assay principle
The HEMOCLOT QuantiV-LKit is a clotting method, triggered by purified Factor Xa, containing Phospholipids and Calcium, and is performed on the diluted tested plasma, in presence of Activated Protein C and Protein S. In the first step, the diluted plasma is mixed with purified clotting Factors (clotting mixture containing Prothrombin, Fibrinogen, Protein S and Activated Protein C (APC)), in a constant and optimised concentration. Then, the purified Factor Xa in presence of Phospholipids (PLP) is added. Clotting is initiated by the addition of Calcium (Ca2+). The clotting time is then recorded. Clottingtimemeasured is inversely proportional to the concentration of factor V-L. There is an inverse linear relationship, on a bilogarithmicgraph paper, between the factor V-L concentration and the corresponding clotting time.
Calibration
The calibration curve can be established with the Factor V-L Calibrator kit (#ASC065K):
Heterozygous FactorV-L Plasma Pool
(50% ofFactorV ⇒V-L)
1/20 ⇒50% V-L
1/10 ⇒100% V-L
For more information please visit www.aniara.com
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- Contact Information
- Jonas Kingo
- V.P of Aniara
- Aniara Corporation
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